Research Assistant in Translational Melanoma Research

Do you want to contribute to top quality medical research?

The Malignant Melanoma Research Group at Karolinska Institutet led by Hildur Helgadottir investigates the biology, genetics and different aspects related to treatment of malignant melanoma. You will join a translational research environment combining clinical samples, cell and molecular biology, extracellular vesicle research and biomarker discovery. Working closely with Postdoctoral Researcher Albano Cáceres Verschae, you will gain experience in laboratory workflows supporting precision oncology and biomarker discovery. The position offers the opportunity to contribute to clinically relevant research within an interdisciplinary and collaborative team at the Department of Oncology-Pathology.

Your mission

We are seeking a motivated Research Assistant to support translational research in malignant melanoma, with a particular focus on extracellular vesicles and circulating biomarkers associated with treatment efficacy.

You will contribute to laboratory studies using melanoma cell models and clinical plasma samples. The work will include extracellular vesicle isolation by ultracentrifugation and size-exclusion chromatography, nanoparticle tracking analysis, mammalian cell culture and downstream molecular characterisation.

You will perform protein and nucleic acid analyses, including western blotting, RT-PCR, immunofluorescence and immunoprecipitation. Additional methods may include ELISA and lentiviral vector production and validation. You will also contribute to data analysis using GraphPad Prism, Qlucore Omics Explorer and R.

You will support the planning and execution of multi-step experiments, maintain accurate laboratory documentation and contribute to laboratory workflow management. The role may also include coordination of research activities, timelines, resources and documentation across collaborative projects.

We are looking for a candidate with:A degree in molecular biology, biomedicine, biotechnology, cell biology or a related disciplinePractical experience in laboratory-based biomedical researchRequired qualifications and experienceA degree in molecular biology, biomedicine, biotechnology, cell biology or a related disciplinePractical experience in mammalian cell cultureExperience with basic molecular and protein biology techniques, including:Western blottingImmunoprecipitationExperience in handling biological samplesAbility to work carefully, independently and collaborativelyGood communication skills in EnglishAbility to maintain accurate laboratory documentationDesirable qualifications and experience

Clinical sample handlingExperience working with human tissue or blood-derived samplesExperience with PBMC isolationMolecular biology techniquesExtracellular vesicle, EV, isolation and characterisationCentrifugation-based EV isolationMicroscopy and image analysisELISA, RT-PCR or related molecular assaysBioinformatics and data analysisExperience with GraphPad Prism, R or other data-analysis toolsParticularly advantageous experienceSize-exclusion chromatography, SEC, based EV isolationNanoparticle tracking analysis, NTA, profilingLentiviral vector production and validationCoordination of experiments or support of laboratory workflowsWhat do we offer?

A creative and inspiring environment with wide-ranging expertise and interests. Karolinska Institutet is one of the world's leading medical universities. Our vision is to pursue the development of knowledge about life and to promote a better health for all. At Karolinska Institutet, we conduct successful medical research and hold the largest range of medical education in Sweden. Karolinska Institutet is also a state university, which entitles you to several good benefits through our collective agreement. And you get to practice freely in our modern wellness facilities, where trained staff are on site.

Location: Department of Oncology-Pathology, Bioclinicum, Solna

Choose to work at KI – Ten reasons why

Application

KI applies individual and differentiated salary setting in accordance with our collective agreements RALS and RALS-T.

The application is to be submitted through the Varbi recruitment system.In this recruitment, you will apply with your CV without a personal letter. Instead, you will answer some questions about why you are applying for the job in the application form. Apply at the latest 15th October.

This is a temporary employment for 6 month.

Want to make a difference? Join us and contribute to better health for all